欧宝在线登录

教师名录

欧宝在线登录:戴隆海

作者:欧宝在线登录发布时间:2022年05月23日 18:26

一、基本信息

戴隆海,博士,副教授,硕士生导师

电子邮件:dailonghai(at)hubu.edu.cn

研究领域:生物催化与酶工程、酶结构与催化机理

二、工作经历

2019.07至今         欧宝在线登录,省部共建生物催化与酶工程国家重点实验室,副教授

2018.10–2020.10  欧宝在线登录,材料科学学院,博士后

2016.07–2018.09  中国科学院天津工业生物技术研究所,工业酶国家工程实验室,助理研究员

三、学习经历

2013.09–2016.07  中国科学院天津工业生物技术研究所,博士

2010.09–2013.03  天津科技大学,硕士

2006.09–2010.07  南昌大学,学士

四、主持科研项目

1. 国家重点研发计划之子课题,144.5万,2021.072024.07,子课题负责人

2. 国家自然科学青年基金,22万,2018.012020.12, 主持

3. 湖北省人才项目经费,20万,2019.062024.05,主持

4. 中国博士后基金面上资助,8万,2019.072020.08,主持

5. 湖北省博士后创新研究岗,6万,2019.072020.08,主持

五、代表性论文(#第一作者; *通讯作者)

1. Li H., #, Yang Y.,# Hu Y.,# Chen, C.C., Huang, J.W., Min, J., Dai, L.,* Guo, R.T.* Structural analysis and engineering of aldo-keto reductase from glyphosate-resistant Echinochloa colona. Journal of Hazardous Materials, 2022. DOI: 10.1016/j.jhazmat.2022.129191.

2. Dai, L.,# Zhang, X.,# Hu Y,# Shen, J., Zhang, Q., Zhang, L., Min, J., Chen C.C., Liu, L.,* Huang, J.W.,* Guo, R.T.* Structural and functional insights into a nonheme iron- and α-ketoglutarate-dependent halogenase that catalyzes chlorination of nucleotide substrates. Applied and Environmental Microbiology, 2022. 88(9).

3. Hu, Y.,# Li, H.,# Min, J.,#, Yu, Y., Liu, W, Huang, J.W., Zhang, L., Yang, Y., Dai, L.,* Chen C.C.,* Guo, R.T.* Crystal structure and biochemical analysis of the specialized deoxynivalenol–detoxifying glyoxalase SPG from Gossypium hirsutum. International Journal of Biological Macromolecules, 2022, 200, 388-396.

4. Chen, C.C.,# Han, X.,# Li, X.,# Jiang, P., Niu, D., Ma, L., Liu, W., Li, S., Qu, Y., Hu, H., Min, J., Yang, Y., Zhang, L., Zeng, W., Huang, J.W.,* Dai, L.,* Guo, R.T.* General features to enhance enzymatic activity of poly(ethylene terephthalate) hydrolysis. Nature Catalysis, 2021, 4, 425-430.

5. Dai, L.,# Qu, Y.,# Hu Y,# Min J, Yu X., Chen, C.C., Huang, J.W.,* Guo, R.T.* Catalytically inactive lytic polysaccharide monooxygenase PcAA14A enhances the enzyme-mediated hydrolysis of polyethylene terephthalate. International Journal of Biological Macromolecules, 2021, 190, 456-462.

6. Dai, L., Hu, Y., Chen, C.C., Ma, L.,* Guo, R.T.* Flavonoid C-glycosyltransferases: function, evolutionary relationship, catalytic mechanism and protein engineering. ChemBioEng Reviews, 2021, 8(1), 15-26.

7. Dai, L.,# Qu, Y.,# Huang, J.W., Hu, Y., Hu, H., Li, S., Chen C.C.,* Guo, R.T.* Enhancing PET hydrolytic enzyme activity by fusion of the cellulose-binding domain of cellobiohydrolase I from Trichoderma reesei. Journal of Biotechnology, 2021, 334: 47-50.

8. Dai, L.,# Qin L.,# Hu, Y., Huang, J. W., Hu, Z., Min, J., Sun, Y.,*, Guo, R.T.,* Structural dissection of unnatural ginsenoside-biosynthetic UDP-glycosyltransferase Bs-YjiC from Bacillus subtilis for substrate promiscuity. Biochemical and Biophysical Research Communications, 2021, 534, 73-78.

9. Chen, C.C.,# Xue, J.,# Peng, W.,# Wang, B., Zhang, L., Liu, W., Ko, T.P., Huang J.W., Zhou, S., Min, J., Ma, L., Dai, L.,* Guo, R.T.,* Yu, X.* Structural insights into thebaine synthase 2 catalysis. Biochemical and Biophysical Research Communications, 2020, 529(2), 156-161.

10. Hu, Y., Li H., Qu Y., Zhang, X., Zhang J., Dai, L.,* Biocatalytic synthesis of a novel bioactive ginsenoside using UDP-glycosyltransferase from Bacillus Subtilis 168. Catalysts, 2020, 10: 289.

11. Hu, Y.,# Min, J.,# Qu, Y.; Zhang, X., Zhang, J.,* Yu, X.,* Dai, L.* Biocatalytic synthesis of calycosin-7-O-β-D-glucoside with UDP-glucose regeneration system. Catalysts, 2020, 10: 258.

12. Hu, Y.,# Xue, J.,# Min, J.,# Qin, L., Zhang, J.,* Dai, L.* Biocatalytic synthesis of ginsenoside Rh2 using Arabidopsis thaliana glucosyltransferase-catalyzed coupled reactions. Journal of Biotechnology, 2020, 309: 107-112.

13. Dai, L.,# Liu, C.,# Li, J., Dong, C., Yang, J., Dai, Z., Zhang, X.,* Sun, Y.* One-pot synthesis of ginsenoside Rh2 and bioactive unnatural ginsenoside by coupling promiscuous glycosyltransferase from Bacillus subtilis 168 to sucrose synthase. Journal of Agricultural and Food Chemistry, 2018, 66: 2830-2837.

14. Dai, L., Li, J., Yang, J., Zhu, Y., Men, Y., Zeng, Y., Cai, Y., Dong, C., Dai, Z., Zhang, X.*, Sun, Y.* Use of a promiscuous glycosyltransferase from Bacillus subtilis 168 for the enzymatic synthesis of novel protopanaxatriol-type ginsenosides. Journal of Agricultural and Food Chemistry, 2018, 66: 943-949.

15. Dai, L.,# Li, J.,# Yang, J., Men, Y., Zeng, Y., Cai, Y., Sun, Y.* Enzymatic synthesis of novel glycyrrhizic acid glucosides using a promiscuous Bacillus glycosyltransferase. Catalysts, 2018, 8: 615.

16. Dai, L., Li, J., Yao, P., Zhu, Y., Men, Y., Zeng, Y., Yang, J., Sun, Y.* Exploiting the aglycon promiscuity of glycosyltransferase Bs-YjiC from Bacillus subtilis and its application in synthesis of glycosides. Journal of Biotechnology, 2017, 248: 69-76.

17. Dai, L., Liu, C., Zhu, Y., Zhang, J., Men, Y., Zeng, Y., Sun, Y.* Functional characterization of cucurbitadienol synthase and triterpene glycosyltransferase involved in biosynthesis of mogrosides from Siraitia grosvenorii. Plant and Cell Physiology, 2015, 56: 1172-1182.

18. Liu, C.,# Dai, L.,# Liu, Y., Dou, D.,* Sun, Y., Ma, L.* Pharmacological activities of mogrosides. Future Medicinal Chemistry, 2018, 10(8):845-850.

19. Zhang, J.,# Dai, L.,# Yang, J., Liu, C., Men, Y., Zeng, Y., Cai, Y., Zhu, Y., Sun, Y.* Oxidation of cucurbitadienol catalyzed by CYP87D18 in the biosynthesis of mogrosides from Siraitia grosvenorii. Plant and Cell Physiology, 2016, 57: 1000-1007.

20. Liu, C.,# Dai, L.,# Dou, D.,* Ma, L.,* Sun, Y. A natural food sweetener with anti-pancreatic cancer properties. Oncogenesis, 2016, 5: 217.

21. Liu, C.,# Zeng, Y.,# Dai, L., # Cai, T., Zhu, Y., Dou, D.,* Ma, L.,* Sun, Y. Mogrol represents a novel leukemia therapeutic, via ERK and STAT3 inhibition. American Journal of Cancer Research, 2015, 5: 1308.

六、申请与授权专利

1. Chen, C. C., Huang, J. W., Dai, L., Yu, X., Chen, C., Wu, S., Zhan, Z., Zhang, L., Zhai, C., Ma, L., Guo, R. T. Cellulase having improved enzymatic activity,授权号:US10479985B1,美国专利。

2. 孙媛霞,戴隆海,李娇,杨建刚,门燕;一种非天然人参皂苷Rd12及其制备方法和应用;授权号:ZL201711146324.6,中国专利。

3. 孙媛霞,戴隆海,张学礼,戴住波,李娇;一种非天然人参皂苷的制备方法及应用;申请号:201711146314.2,中国专利。

4. 孙媛霞,戴隆海,张学礼、戴住波,李娇;一组天然与非天然的原人参三醇型人参皂苷的合成方法;申请号:201711155328.0,中国专利。

5. 孙媛霞,戴隆海,朱玥明,曾艳,门燕,杨建刚;一种稀有人参皂苷Rh2的生产方法;申请号:CN201610815471,中国专利。

6. 肖冬光,张翠英,郭学武,张建炜,戴隆海;一株高产乙酸酯酿酒酵母工程菌;授权号:ZL201210080231,中国专利。

7. 肖冬光,张翠英,郭学武,张建炜,戴隆海;一株含有Lg-ATF1基因的酿酒酵母工程菌;授权号:ZL201210080040,中国专利。

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